PrimePCR™ SYBR® Green Assay: APOLD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004405
Assay Design:   Exonic
Chromosome Location:   12:12942474-12942575question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000326765 ENST00000356591

Gene Information

APOLD1 is an endothelial cell early response protein that may play a role in regulation of endothelial cell signaling and vascular function (Regard et al. 2004 [PubMed 15102925]).[supplied by OMIM Dec 2008]

Gene Symbol:   APOLD1
Gene Name:   apolipoprotein L domain containing 1
Aliases:   DKFZp434F0318, FLJ25138, FLJ95166, VERGE
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000178878
Entrez:   81575
Chromosome Mapping:   12p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.230000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download