This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: CD59, Human
PrimePCR™ Template for SYBR® Green Assay: CD59, Human
This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex whereby it binds complement C8 and/or C9 during the assembly of this complex thereby inhibiting the incorporation of multiple copies of C9 into the complex which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency a disease resulting in hemolytic anemia and thrombosis and which causes cerebral infarction. Multiple alternatively spliced transcript variants which encode the same protein have been identified for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.