PrimePCR™ SYBR® Green Assay: POU6F2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: POU6F2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: POU6F2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004040
Assay Design:   Exonic
Chromosome Location:   7:39504116-39504278question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000403058 ENST00000518318

Gene Information

This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain consisting of a POU-specific domain and a homeodomain separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers in a sequence-specific manner. The POU family members are transcriptional regulators many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq Oct 2009]

Gene Symbol:   POU6F2
Gene Name:   POU class 6 homeobox 2
Aliases:   RPF-1, WT5, WTSL
RefSeq:   NC_000007.13 NT_007819.17 NG_016022.1
Ensembl:   ENSG00000106536
Entrez:   11281
Chromosome Mapping:   7p14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.390000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download