PrimePCR™ SYBR® Green Assay: WFS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: WFS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: WFS1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003817
Assay Design:   Exonic
Chromosome Location:   4:6303464-6303598question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000503569 ENST00000226760 ENST00000540337

Gene Information

This gene encodes a transmembrane protein which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain pancreas heart and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome also called DIDMOAD (Diabetes Insipidus Diabetes Mellitus Optic Atrophy and Deafness) an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6) also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Mar 2009]

Gene Symbol:   WFS1
Gene Name:   Wolfram syndrome 1 (wolframin)
Aliases:   FLJ51211, WFRS, WFS
RefSeq:   NC_000004.11 NT_006051.18 NG_011700.1
Ensembl:   ENSG00000109501
Entrez:   7466
Chromosome Mapping:   4p16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.290000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download