PrimePCR™ SYBR® Green Assay: CTLA4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024830

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CTLA4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CTLA4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003794
Assay Design:   Exonic
Chromosome Location:   2:204735462-204735607question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000302823 ENST00000295854 ENST00000427473

Gene Information

This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain a transmembrane domain and a cytoplasmic tail. Alternate transcriptional splice variants encoding different isoforms have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus Graves disease Hashimoto thyroiditis celiac disease systemic lupus erythematosus thyroid-associated orbitopathy and other autoimmune diseases. [provided by RefSeq Jul 2008]

Gene Symbol:   CTLA4
Gene Name:   cytotoxic T-lymphocyte-associated protein 4
Aliases:   CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, ICOS, IDDM12
RefSeq:   NC_000002.11 NT_005403.17 NG_011502.1
Ensembl:   ENSG00000163599
Entrez:   1493
Chromosome Mapping:   2q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.360000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download