PrimePCR™ SYBR® Green Assay: HOXC4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: HOXC4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HOXC4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003770
Assay Design:   Exonic
Chromosome Location:   12:54448672-54448775question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000303406 ENST00000430889

Gene Information

This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters HOXA HOXB HOXC and HOXD which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene HOXC4 is one of several homeobox HOXC genes located in a cluster on chromosome 12. Three genes HOXC5 HOXC4 and HOXC6 share a 5' non-coding exon. Transcripts may include the shared exon spliced to the gene-specific exons or they may include only the gene-specific exons. Two alternatively spliced variants that encode the same protein have been described for HOXC4. Transcript variant one includes the shared exon and transcript variant two includes only gene-specific exons. [provided by RefSeq Jul 2008]

Gene Symbol:   HOXC4
Gene Name:   homeobox C4
Aliases:   HOX3, HOX3E, cp19
RefSeq:   NC_000012.11 NG_005542.1 NT_029419.12
Ensembl:   ENSG00000198353
Entrez:   3221
Chromosome Mapping:   12q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.620000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download