PrimePCR™ SYBR® Green Assay: MANF, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: MANF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MANF, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003210
Assay Design:   Exonic
Chromosome Location:   3:51425175-51425307question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000528157 ENST00000273628

Gene Information

The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of polymorphisms in the arginine-rich region including a specific mutation that changes the previously numbered codon 50 from ATG to AGG have been reported in a variety of solid tumors; however these polymorphisms were later shown to exist in normal tissues and are thus not tumor-related. [provided by RefSeq Jun 2010]

Gene Symbol:   MANF
Gene Name:   mesencephalic astrocyte-derived neurotrophic factor
Aliases:   ARMET, ARP, MGC142148, MGC142150
RefSeq:   NC_000003.11 NG_012652.2 NG_028012.1 NT_022517.18 NW_003315910.1
Ensembl:   ENSG00000145050
Entrez:   7873
Chromosome Mapping:   3p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.960000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download