PrimePCR™ SYBR® Green Assay: LALBA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024476

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LALBA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LALBA, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002690
Assay Design:   Exonic
Chromosome Location:   12:48962872-48962989question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000301046 ENST00000549817

Gene Information

This gene encodes alpha-lactalbumin a principal protein of milk. Alpha-lactalbumin forms the regulatory subunit of the lactose synthase (LS) heterodimer and beta 14-galactosyltransferase (beta4Gal-T1) forms the catalytic component. Together these proteins enable LS to produce lactose by transfering galactose moieties to glucose. As a monomer alpha-lactalbumin strongly binds calcium and zinc ions and may possess bactericidal or antitumor activity. A folding variant of alpha-lactalbumin called HAMLET likely induces apoptosis in tumor and immature cells. [provided by RefSeq Jul 2008]

Gene Symbol:   LALBA
Gene Name:   lactalbumin, alpha-
Aliases:   MGC138521, MGC138523
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000167531
Entrez:   3906
Chromosome Mapping:   12q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 35.250000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download