PrimePCR™ SYBR® Green Assay: NADK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002548
Assay Design:   Exonic
Chromosome Location:   1:1682990-1683164question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000341426 ENST00000341991 ENST00000378625 ENST00000344463

Gene Information

NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al. 2001 [PubMed 11594753]).[supplied by OMIM Mar 2008]

Gene Symbol:   NADK
Gene Name:   NAD kinase
Aliases:   DKFZp686L22239, FLJ13052, FLJ37724, FLJ54695, FLJ77769, FLJ78247, FLJ78307, MGC1900, dJ283E3.1
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000008130
Entrez:   65220
UniGene:   Hs.654792
Chromosome Mapping:   1p36.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.940000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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