PrimePCR™ SYBR® Green Assay: CAP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024352

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CAP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CAP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002349
Assay Design:   Exonic
Chromosome Location:   1:40527407-40529925question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000538246 ENST00000424977 ENST00000372797 ENST00000372802 ENST00000449311 ENST00000421589 ENST00000414281 ENST00000420216 ENST00000372792 ENST00000372798 ENST00000340450 ENST00000372805 ENST00000435719 ENST00000427843 ENST00000417287

Gene Information

The protein encoded by this gene is related to the S. cerevisiae CAP protein which is involved in the cyclic AMP pathway. The human protein is able to interact with other molecules of the same protein as well as with CAP2 and actin. Alternatively spliced transcript variants have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   CAP1
Gene Name:   CAP, adenylate cyclase-associated protein 1 (yeast)
Aliases:   CAP, CAP1-PEN
RefSeq:   NC_000001.10 NG_009192.1 NT_032977.9
Ensembl:   ENSG00000131236
Entrez:   10487
Chromosome Mapping:   1p34.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.510000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download