PrimePCR™ SYBR® Green Assay: TPM2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024289

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TPM2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TPM2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002140
Assay Design:   Exonic
Chromosome Location:   9:35689704-35689837question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000378300 ENST00000378292 ENST00000329305 ENST00000360958

Gene Information

This gene encodes beta-tropomyosin a member of the actin filament binding protein family and mainly expressed in slow type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins and cause cap disease nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Mar 2009]

Gene Symbol:   TPM2
Gene Name:   tropomyosin 2 (beta)
Aliases:   AMCD1, DA1, DA2B, NEM4, TMSB
RefSeq:   NC_000009.11 NT_008413.18 NG_011620.1
Ensembl:   ENSG00000198467
Entrez:   7169
Chromosome Mapping:   9p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.010000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download