PrimePCR™ SYBR® Green Assay: SETDB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024034

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001355
Assay Design:   Exonic
Chromosome Location:   1:150900222-150900332question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000271640 ENST00000448029 ENST00000368962 ENST00000534805 ENST00000368969 ENST00000368963 ENST00000498193 ENST00000423081 ENST00000413562 ENST00000525956

Gene Information

This gene encodes a histone methyltransferase which regulates histone methylation gene silencing and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.[provided by RefSeq Jun 2011]

Gene Symbol:   SETDB1
Gene Name:   SET domain, bifurcated 1
Aliases:   ESET, H3-K9-HMTase4, KG1T, KIAA0067, KMT1E
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000143379
Entrez:   9869
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.150000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download