PrimePCR™ SYBR® Green Assay: SLC20A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024009

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC20A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC20A1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001284
Assay Design:   Exonic
Chromosome Location:   2:113417079-113417200question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000272542 ENST00000409095

Gene Information

The protein encoded by this gene is a sodium-phosphate symporter that absorbs phosphate from interstitial fluid for use in cellular functions such as metabolism signal transduction and nucleic acid and lipid synthesis. The encoded protein is also a retroviral receptor causing human cells to be susceptible to infection by gibbon ape leukemia virus simian sarcoma-associated virus feline leukemia virus subgroup B and 10A1 murine leukemia virus.[provided by RefSeq Mar 2011]

Gene Symbol:   SLC20A1
Gene Name:   solute carrier family 20 (phosphate transporter), member 1
Aliases:   DKFZp686J2397, FLJ41426, GLVR1, Glvr-1, PIT1, PiT-1
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000144136
Entrez:   6574
Chromosome Mapping:   2q11-q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.650000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download