PrimePCR™ SYBR® Green Assay: FIBCD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001236
Assay Design:   Exonic
Chromosome Location:   9:133799131-133799220question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000372338 ENST00000372337 ENST00000444139 ENST00000451466 ENST00000253018 ENST00000448616 ENST00000454493

Gene Information

FIBCD1 is a conserved type II transmembrane endocytic receptor that binds chitin and is located primarily in the intestinal brush border (Schlosser et al. 2009 [PubMed 19710473]).[supplied by OMIM Apr 2010]

Gene Symbol:   FIBCD1
Gene Name:   fibrinogen C domain containing 1
Aliases:   FLJ14810
RefSeq:   NC_000009.11 NT_035014.4
Ensembl:   ENSG00000130720
Entrez:   84929
Chromosome Mapping:   9q34.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.170000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download