PrimePCR™ SYBR® Green Assay: PEG10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0023986

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001214
Assay Design:   Exonic
Chromosome Location:   7:94292864-94292967question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000482108 ENST00000488574

Gene Information

This is a paternally expressed imprinted gene that encodes transcripts containing two overlapping open reading frames (ORFs) RF1 and RF1/RF2 as well as retroviral-like slippage and pseudoknot elements which can induce a -1 nucleotide frame-shift. ORF1 encodes a shorter isoform with a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons. The longer isoform is the result of -1 translational frame-shifting leading to translation of a gag/pol-like protein combining RF1 and RF2. It contains the active-site consensus sequence of the protease domain of pol proteins. Additional isoforms resulting from alternatively spliced transcript variants as well as from use of upstream non-AUG (CUG) start codon have been reported for this gene. Increased expression of this gene is associated with hepatocellular carcinomas. [provided by RefSeq May 2010]

Gene Symbol:   PEG10
Gene Name:   paternally expressed 10
Aliases:   EDR, HB-1, KIAA1051, MEF3L, Mar2, Mart2, RGAG3
RefSeq:   NC_000007.13 NG_008893.1 NG_011340.1 NT_007933.15
Ensembl:   ENSG00000242265
Entrez:   23089
UniGene:   Hs.147492
Chromosome Mapping:   7q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.140000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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