PrimePCR™ SYBR® Green Assay: TCN2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001102
Assay Design:   Exonic
Chromosome Location:   22:31013317-31013474question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000215838 ENST00000405742 ENST00000407817

Gene Information

This gene encodes a member of the vitamin B12-binding protein family. This family of proteins alternatively referred to as R binders is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins such as transcobalamin I and gastric intrisic factor may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.[provided by RefSeq May 2010]

Gene Symbol:   TCN2
Gene Name:   transcobalamin II
Aliases:   D22S676, D22S750, II, TC, TC II, TC-2, TC2, TCII
RefSeq:   NC_000022.10 NG_007263.1 NT_011520.12
Ensembl:   ENSG00000185339
Entrez:   6948
UniGene:   Hs.417948
Chromosome Mapping:   22q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.170000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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