PrimePCR™ SYBR® Green Assay: LRP5L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0023918

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001014
Assay Design:   Exonic
Chromosome Location:   22:25747525-25747699question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000444995 ENST00000402785 ENST00000402859

Gene Information

Description Not Available

Gene Symbol:   LRP5L
Gene Name:   low density lipoprotein receptor-related protein 5-like
Aliases:   DKFZp434O0213
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100068
Entrez:   91355
UniGene:   Hs.634058
Chromosome Mapping:   22q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.810000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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