PrimePCR™ Probe Assay: CHLM, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CHLM, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CHLM, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0026520
Assay Design:   Exonic
Chromosome Location:   4:12877367-12877764question
Amplicon Length:   193
Splice Variants Targeted:   AT5G46290.1 AT5G46290.3 AT4G25080.1 AT4G25080.2 AT4G25080.3 AT4G25080.4 AT4G25080.5

Gene Information

Encodes a protein with methyltransferase activity responsible for the methylation of magnesium protoporphyrin IX. Mutants defective in this gene are affected in chlorophyll biosynthesis and show a reduction in the accumulation of a number of major thylakoid-associated proteins including components of PSI (LHCI), PSII (LHCII, D1, CP43) and the cytochrome b6f complex (Cytf). By contrast, no significant changes were detected for the proteins of the stroma and the chloroplast envelope.

Gene Symbol:   CHLM
Gene Name:   Magnesium-protoporphyrin IX methyltransferase
Aliases:   Not Available
RefSeq:   NM_001203903 NM_179108 NM_118640 NM_001125580 NM_179107
Ensembl:   AT4G25080
Entrez:   828611
Chromosome Mapping:   Not Available

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