PrimePCR™ Probe Assay: ASN1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ASN1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ASN1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0025735
Assay Design:   Exonic
Chromosome Location:   3:17438162-17438322question
Amplicon Length:   131
Splice Variants Targeted:   AT3G47340.1 AT3G47340.2 AT3G47340.3 AT2G26690.1

Gene Information

encodes a glutamine-dependent asparagine synthetase, the predicted ASN1 peptide contains a purF-type glutamine-binding domain, and is expressed predominantly in shoot tissues, where light has a negative effect on its mRNA accumulation. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.

Gene Symbol:   ASN1
Gene Name:   Glutamine-dependent asparagine synthase 1
Aliases:   Not Available
RefSeq:   NM_114602 NM_180332 NM_180333
Ensembl:   AT3G47340
Entrez:   823888
Chromosome Mapping:   Not Available

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