PrimePCR™ Probe Assay: FRD3, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FRD3, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FRD3, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0025227
Assay Design:   Exonic
Chromosome Location:   3:2568891-2569038question
Amplicon Length:   118
Splice Variants Targeted:   AT5G56000.1 AT5G56010.1 AT3G08040.1 AT3G08040.2

Gene Information

Encodes a member of the MATE (multidrug and toxin efflux family), expressed in roots but not shoots. Mutants accumulate excess iron, manganese and zinc, and express root Fe(III) chelatase activity even under iron sufficiency conditions. FRD3 is likely to function in root xylem loading of an iron chelator or other factor necessary for efficient iron uptake out of the xylem or apoplastic space and into leaf cells.

Gene Symbol:   FRD3
Gene Name:   MATE efflux family protein
Aliases:   Not Available
RefSeq:   NM_111683 NM_001161123
Ensembl:   AT3G08040
Entrez:   819995
Chromosome Mapping:   Not Available

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