PrimePCR™ Probe Assay: BOR1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BOR1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BOR1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0025061
Assay Design:   Exonic
Chromosome Location:   2:19358666-19358761question
Amplicon Length:   66
Splice Variants Targeted:   AT3G62270.1 AT2G47160.1 AT2G47160.2

Gene Information

Boron transporter. Protein accumulates in shoots and roots under conditions of boron deficiency and is degraded within several hours of restoring boron supply. Localized to the plasma membrane under B limitation, and to the cytoplasm after B application before degradation. Protein is transferred via the endosomes to the vacuole for degradation. Localized to the inner plasma membrane domain in the columella, lateral root cap, epidermis, and endodermis in the root tip region, and in the epidermis and endodermis in the elongation zone. Under high-boron is transported to the vacuole for degradation.

Gene Symbol:   BOR1
Gene Name:   HCO3- transporter family
Aliases:   Not Available
RefSeq:   NM_001084602 NM_180138
Ensembl:   AT2G47160
Entrez:   819329
Chromosome Mapping:   Not Available

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