PrimePCR™ Probe Assay: IMB1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IMB1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IMB1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0024790
Assay Design:   Exonic
Chromosome Location:   2:14724274-14724394question
Amplicon Length:   91
Splice Variants Targeted:   AT5G19220.1 AT2G34900.1 AT2G34900.2

Gene Information

Encodes a member of the BET subgroup of bromodomain proteins, a novel class of putative transcription factors. Its expression is induced during seed imbibition and downregulated during germination. Seeds of a loss-of-function mutant allele, imb1, show impaired cotyledon greening during germination in abscisic acid (ABA) and express higher levels of ABI5 protein than the wild type. Moreover, imb1 seeds are deficient in the phytochrome A (phyA)-mediated very-low-fluence response of germination.

Gene Symbol:   IMB1
Gene Name:   Transcription factor GTE6
Aliases:   Not Available
RefSeq:   NM_129043 NM_201873
Ensembl:   AT2G34900
Entrez:   818055
Chromosome Mapping:   Not Available

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