PrimePCR™ Probe Assay: PIN4, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PIN4, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PIN4, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0024241
Assay Design:   Exonic
Chromosome Location:   2:180344-180491question
Amplicon Length:   118
Splice Variants Targeted:   AT5G46350.1 AT2G01420.1 AT2G01420.2 AT2G36850.1 AT1G15530.1

Gene Information

Encodes a putative auxin efflux carrier that is localized in developing and mature root meristems. It is involved in the maintenance of embryonic auxin gradients. A role for AtPIN4 in generating a sink for auxin below the quiescent center of the root meristem that is essential for auxin distribution and patterning is proposed. In the root, PIN4 is detected around the quiescent center and cells surrounding it, and localizes basally in provascular cells. PIN4 expression is upregulated in brassinosteroid-insensitive mutant (PMID 16141452).

Gene Symbol:   PIN4
Gene Name:   Auxin efflux carrier family protein
Aliases:   Not Available
RefSeq:   NM_126203 NM_179592
Ensembl:   AT2G01420
Entrez:   814670
Chromosome Mapping:   Not Available

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