PrimePCR™ Probe Assay: HGPT, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HGPT, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HGPT, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0024042
Assay Design:   Exonic
Chromosome Location:   1:26988933-26989023question
Amplicon Length:   61
Splice Variants Targeted:   AT5G47560.1 AT1G71750.1 AT1G71750.2

Gene Information

Encodes a protein with hypoxanthine-guanine-phosphoribosyltransferase activity. Unlike some related enzymes, it does not appear to act on xanthine in vitro. The enzyme catalyzes reactions occurring in both directions, but appears to prefer acting on guanine, followed by hypoxanthine, in vitro. The enzyme is likely to function in purine salvage pathways and appears to be important for seed germination.

Gene Symbol:   HGPT
Gene Name:   Hypoxanthine-guanine phosphoribosyltransferase
Aliases:   Not Available
RefSeq:   NM_001198443 NM_105833
Ensembl:   AT1G71750
Entrez:   843505
Chromosome Mapping:   Not Available

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