PrimePCR™ Probe Assay: DDL, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DDL, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DDL, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0016522
Assay Design:   Exonic
Chromosome Location:   3:7177260-7177376question
Amplicon Length:   87
Splice Variants Targeted:   AT3G20550.1

Gene Information

Encodes a nuclear localized FHA (forhkead) domain containing protein.Mutant plants have shortened roots, delayed flowering time, altered floral organ number, defective floral organs and reduced fertility.Ddl mutants also show reduced levels of pri-miRNAs as well as mature miRNAs suggesting involvement in biogenesis of miRNAs. DDL does not affect transcription of miRNAs directly but may act through other proteins such as DCL.

Gene Symbol:   DDL
Gene Name:   SMAD/FHA domain-containing protein
Aliases:   Not Available
RefSeq:   NM_112947
Ensembl:   AT3G20550
Entrez:   821601
Chromosome Mapping:   Not Available

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