PrimePCR™ SYBR® Green Assay: OASA1, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: OASA1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: OASA1, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0053459
Assay Design:   Exonic
Chromosome Location:   4:8519824-8520019question
Amplicon Length:   79
Splice Variants Targeted:   AT5G28020.1 AT5G28020.2 AT5G28020.3 AT5G28020.4 AT5G28020.6 AT4G14880.1 AT4G14880.2 AT4G14880.3 AT4G14880.4

Gene Information

Encodes a cytosolic isoform of cytosolic O-acetylserine(thiol)lyase, a key enzyme in cysteine biosynthesis and for the fixation of inorganic sulfide. It catalyzes the formation of cysteine from O-acetylserine and inorganic sulfide. Gene expression is predominant in the root cortex and the xylem parenchyma. Gene expression is induced in leave, stems and roots by high salt and heavy metal stresses, mediated by ABA. Lines carrying semi-dominant mutations exhibit early senescence.

Gene Symbol:   OASA1
Gene Name:   O-acetylserine (thiol) lyase (OAS-TL) isoform A1
Aliases:   Not Available
RefSeq:   NM_117574 NM_179055 NM_001203803 NM_001203804
Ensembl:   AT4G14880
Entrez:   827145
Chromosome Mapping:   Not Available

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download