PrimePCR™ SYBR® Green Assay: ICE1, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ICE1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ICE1, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0052741
Assay Design:   Exonic
Chromosome Location:   3:9833982-9834131question
Amplicon Length:   120
Splice Variants Targeted:   AT3G11960.1 AT3G11960.2 AT3G26744.1 AT3G26744.2 AT3G26744.4 AT3G58760.1

Gene Information

Encodes a MYC-like bHLH transcriptional activator that binds specifically to the MYC recognition sequences in the CBF3 promoter. Mutants are defective in cold-regulated gene expression. Cold stress triggers protein degradation of nuclear GFPICE1 protein, and the RING finger protein HOS1 is required. Sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance.

Gene Symbol:   ICE1
Gene Name:   Basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Aliases:   Not Available
RefSeq:   NM_113586 NM_001035697 NM_001035699
Ensembl:   AT3G26744
Entrez:   822287
Chromosome Mapping:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download