PrimePCR™ SYBR® Green Assay: IBM1, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0052377
Assay Design:   Exonic
Chromosome Location:   3:2428278-2428416question
Amplicon Length:   109
Splice Variants Targeted:   AT3G07610.1 AT3G07610.2 AT3G07610.3 AT2G27260.1

Gene Information

IBM1 likely encodes a protein with histone H3mK9 demethylation activity. It may preferentially demethylate H3mK9 at low-copy loci to protect them from silencing by nearby heterochromatin by preventing the spread of cytosine methylation. BONSAI (At1g73177) is hypermethylated in ibm1 mutants. ibm1 mutants have morphological defects that become apparent at the F3 generation, including small narrow leaves, arrested flower development, and faulty pollen development. These phenotypes cannot result solely from the BONSAI hypermethylation. Aberrant phenotypes in ibm1 mutants in both DNA methylation and plant development can be suppressed by mutations in the KYP H3K9 methyltransferase or he CMT3 non CG-cytosine methylase.

Gene Symbol:   IBM1
Gene Name:   Transcription factor jumonji (jmjC) domain-containing protein
Aliases:   Not Available
RefSeq:   NM_001202909 NM_001084652 NM_111640
Ensembl:   AT3G07610
Entrez:   819952
UniGene:   At.18334
Chromosome Mapping:   Not Available

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