PrimePCR™ SYBR® Green Assay: TFIIIA, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0051207
Assay Design:   Exonic
Chromosome Location:   1:27116156-27116278question
Amplicon Length:   93
Splice Variants Targeted:   AT4G16530.1 AT1G72050.1 AT1G72050.2

Gene Information

Encodes a transcriptional factor TFIIIA required for transcription of 5S rRNA gene. 5S rRNA is the smallest constituent of the ribosome. Work on one of the gene models AT1G72050.2 showed that it encodes a protein with nine Cys(2)-His(2)-type zinc fingers, a characteristic feature of TFIIIA proteins. AT1G72050.2 also contains a 23 amino acid spacer between fingers 1 and 2, a 66 amino acid spacer between fingers 4 and 5, and a 50 amino acid non-finger C-terminal tail. in vitro assay demonstrated that AT1g72050.2 binds to 5S rDNA and efficiently stimulates the transcription of 5S rRNA. AT1g72050.2 also binds to 5S rRNA in vitro. AT1g72050.2 is located at several nuclear foci including the nucleolus and is absent from the cytoplasm.

Gene Symbol:   TFIIIA
Gene Name:   Transcription factor IIIA
Aliases:   Not Available
RefSeq:   NM_105863 NM_202399
Ensembl:   AT1G72050
Entrez:   843536
UniGene:   At.17480,At.66854
Chromosome Mapping:   Not Available

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