PrimePCR™ SYBR® Green Assay: GalAK, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: GalAK, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GalAK, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0043451
Assay Design:   Exonic
Chromosome Location:   3:3350876-3350975question
Amplicon Length:   70
Splice Variants Targeted:   AT3G10700.1

Gene Information

Encodes a GHMP kinase family protein that acts as a galacturonic acid-1-phosphate kinase that catalyzes the production of galacturonic acid-1-phosphate. This is a precursor of the important cell wall building block UDP-galacturonic acid. Based on gene trap line GT8007, the gene appears to be expressed in a petal and stamen-specific manner, between flower stages 8 to 11, however, later RT-qPCR analysis demonstrates that the transcript is present throughout the plant in all tissues tested.

Gene Symbol:   GalAK
Gene Name:   Galacturonic acid kinase
Aliases:   Not Available
RefSeq:   NM_111906
Ensembl:   AT3G10700
Entrez:   820239
Chromosome Mapping:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download