PrimePCR™ SYBR® Green Assay: KCR2, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: KCR2, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KCR2, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0040824
Assay Design:   Exonic
Chromosome Location:   1:8674263-8674386question
Amplicon Length:   94
Splice Variants Targeted:   AT1G24470.1

Gene Information

Encodes one of the two Arabidopsis homologues to YBR159w encoding a S. cerevisiae beta-ketoacyl reductase (KCR), which catalyzes the first reduction during VLCFA (very long chain fatty acids, >18 carbon) elongation: KCR1 (At1g67730), KCR2 (At1g24470). Complementation of the yeast ybr159Delta mutant demonstrated that the two KCR proteins are divergent and that only AtKCR1 can restore heterologous elongase activity similar to the native yeast KCR gene.

Gene Symbol:   KCR2
Gene Name:   Beta-ketoacyl reductase 2
Aliases:   Not Available
RefSeq:   NM_102292
Ensembl:   AT1G24470
Entrez:   839063
Chromosome Mapping:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download