PrimePCR™ SYBR® Green Assay: AGL61, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: AGL61, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AGL61, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0032274
Assay Design:   Exonic
Chromosome Location:   2:10581621-10581746question
Amplicon Length:   96
Splice Variants Targeted:   AT2G24840.1

Gene Information

Encodes a member of the Agamous-like family of transcription factors. Localized to the nucleus in the central cell and endosperm of the female gametophyte. Loss of function mutations show reduced female fertility. Fifty percent of ovules have defective central cells with abnormal morphology and patterns of gene expression. Upon fertilization 50% of seeds abort. Using yeast two hybrid assays AGL61 was shown to interact with AGL80, another MADS box gene with similar defects in ovule development. These data suggest that AGL61 and 80 together are required for proper differentiation of the central cell.

Gene Symbol:   AGL61
Gene Name:   AGAMOUS-like 61
Aliases:   Not Available
RefSeq:   NM_179727
Ensembl:   AT2G24840
Entrez:   817021
Chromosome Mapping:   Not Available

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download