PrimePCR™ SYBR® Green Assay: ACS1, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ACS1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ACS1, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0030477
Assay Design:   Exonic
Chromosome Location:   3:22764376-22764916question
Amplicon Length:   98
Splice Variants Targeted:   AT3G61510.1

Gene Information

Encodes a member of the 1-aminocyclopropane-1-carboxylate (ACC) synthase (S-adenosyl-L-methionine methylthioadenosine-lyase, EC 4.4.1.14) gene family. The gene is transcriptionally active but enzymatically inactive. The predicted amino-acid sequence of ACS1 is missing the highly conserved tripeptide, Thr-Asn-Pro (TNP), between Ile204 and Ser205. Introduction of TNP into ACS1 restores the ACS activity.

Gene Symbol:   ACS1
Gene Name:   ACC synthase 1
Aliases:   Not Available
RefSeq:   NM_116016
Ensembl:   AT3G61510
Entrez:   825324
Chromosome Mapping:   Not Available

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download