PrimePCR™ SYBR® Green Assay: SPO11-2, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0030263
Assay Design:   Exonic
Chromosome Location:   1:23744263-23744465question
Amplicon Length:   95
Splice Variants Targeted:   AT1G63990.1

Gene Information

Encodes AtSPO11-2, one of the three Arabidopsis homologues of the archaeal DNA topoisomerase VIA subunit (topo VIA). Required for meiotic recombination. Plants homozygous for atspo11-2 exhibit a severe sterility phenotype. Both male and female meiosis are severely disrupted in the atspo11-2 mutant, and this is associated with severe defects in synapsis during the first meiotic division and reduced meiotic recombination. AtSPO11-1 and AtSPO11-2 have overlapping functions (i.e. both required for meiotic recombination) whereas AtSPO11-3 functions in DNA replication. Required for double-strand break induction.

Gene Symbol:   SPO11-2
Gene Name:   Sporulation 11-2
Aliases:   Not Available
RefSeq:   NM_105072
Ensembl:   AT1G63990
Entrez:   842702
UniGene:   At.48342
Chromosome Mapping:   Not Available

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