PrimePCR™ SYBR® Green Assay: OTP51, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0029818
Assay Design:   Exonic
Chromosome Location:   2:6890913-6891012question
Amplicon Length:   70
Splice Variants Targeted:   AT2G15820.1

Gene Information

Encodes a protein that promotes splicing of type II introns. otp51 mutants fail to splice intron 2 of plastid ycf3 transcripts, a factor required for the assembly of Photosystem I. Therefore, homozygous otp51 mutants have profound photosynthetic defects and can only survive in sucrose-supplemented in vitro cultures under low light conditions. OTP51 may also be involved in splicing several other transcripts and precursor forms of the trnL, trnG, trnI, and trnA transcripts also accumulate in otp51 mutants. Although OTP51 shares some homology with DNA endonucleases, it lacks key catalytic residues suggesting that it does not participate in DNA cleavage.

Gene Symbol:   OTP51
Gene Name:   Endonucleases
Aliases:   Not Available
RefSeq:   NM_127144
Ensembl:   AT2G15820
Entrez:   816078
UniGene:   At.13866
Chromosome Mapping:   Not Available

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