PrimePCR™ SYBR® Green Assay: CPK6, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0029405
Assay Design:   Exonic
Chromosome Location:   2:7517101-7517250question
Amplicon Length:   120
Splice Variants Targeted:   AT2G17290.1

Gene Information

Encodes calcium dependent protein kinase 6 (CPK6), a member of the Arabidopsis CDPK gene family. CDPKs contain an intrinsic Ca2+-activation domain with four EF hand Ca2+-binding sites. CDPKs protein kinases have been proposed to function in multiple plant signal transduction pathways downstream of [Ca2+]cyt elevations, thus transducing various physiological responses. CPK6 is expressed in both guard cells and mesophyll cells. Functions in guard cell ion channel regulation. ABA and Ca(2+) activation of slow-type anion channels and, interestingly, ABA activation of plasma membrane Ca(2+)-permeable channels were impaired in independent alleles of single and double cpk3cpk6 mutant guard cells. Furthermore, ABA- and Ca(2+)-induced stomatal closing were partially impaired in these cpk3cpk6 mutant alleles. CPK3 is also a member of the Arabidopsis CDPK family.

Gene Symbol:   CPK6
Gene Name:   Calcium-dependent protein kinase family protein
Aliases:   Not Available
RefSeq:   NM_127284
Ensembl:   AT2G17290
Entrez:   816235
UniGene:   At.9828
Chromosome Mapping:   Not Available

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