PrimePCR™ Variant Mutation Detection Assay: ORF1ab T265I, SARS-CoV-2

PrimePCR

The PrimePCR SARS-CoV-2 Single Mutation Assay are single-tube mutation detection assays designed to detect specific mutations in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome. Assays contain unlabeled PCR primers and different dye-labeled, allele-specific probes plus a primer and probe combination for internal positive control.

Info:   Assay differentiates between reference and T265I allele and includes an internal amplification control primer/probe assay.

 
Fluorophore:   FAM,HEX,CY5
List Price:    $889.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Variant Mutation Detection
Unique Assay ID:   qSC2Mut0020212
Amplicon Length:   77
Nucleotide Mutation Detection:   1059C>T
Amino Acid Change:   T265I
MIQE Context :question   TCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACA[C/T]CTTCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAA

Gene Information

The ORF1ab gene is a 21290 nucleotide sequence containing overlapping open reading frames that encode many non-structural proteins essential for replicating and transcribing the viral genome.

Gene Symbol:   ORF1ab
Gene Name:   ORF1ab
Aliases:   ORF1a polyprotein;ORF1ab polyprotein
RefSeq:   NC_045512.2
Ensembl:   ENSSASG00005000002
Entrez:   43740578

Type :   gBlock Gene Fragments
For instructions on preparing the positive controls, please see the assay instruction manual.
Sequence :  

Click Here to select the positive control sequence. Then to copy it to your clipboard, press Ctrl + C for Windows users or ⌘ + C for Mac users.
   
Copy the above positive control sequence and go to the link below and paste to order.
Order from IDT:   https://www.idtdna.com/site/Order/gblockentry
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