This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qRnoCID0004911; intron_spanning
transcription factor; member of the C2H2-type zinc finger protein subclass of the Gli family of transcription factors; mediates sonic hedgehog (Shh) signalling; may play a role in embryogenesis [RGD, Feb 2006]