This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qRnoCID0004524; intron_spanning
increases the activity of squalene epoxidase, which catalyzes the conversion of squalene to squalene 2,3-epoxide in cholesterol biosynthesis; may act as a cytosolic squalene transfer protein [RGD, Feb 2006]