This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0015430; Intron-spanning
This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis whereby it catalyzes the transfer of a guanido group from L-arginine to glycine resulting in guanidinoacetic acid the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency an inborn error of creatine synthesis characterized by mental retardation language impairment and behavioral disorders. [provided by RefSeq Jul 2008]