This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0011079; Intron-spanning
Three phosphofructokinase isozymes exist in humans: muscle liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase which catalyzes the phosphorylation of fructose-6-phosphate to fructose-16-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq Nov 2009]