This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0056842; exonic
The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain 2 tandem repeats of EF-hand calcium-binding motifs and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]