This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0043760; exonic
This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains both produced from a single protein precursor. This proteinase which is a member of the peptidase C1 family has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases including breast cancer and possibly Alzheimer disease. [provided by RefSeq Jul 2008]