PrimePCR™ ddPCR™ Expression Probe Assay: GTF2A2, Human


ddPCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0029009; Intron-spanning

Fluorophore:   HEX
List Price:    $207.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   dHsaCPE5051643
Chromosome Location:   15:59942889-59944504question
Amplicon Length:   154

Gene Information

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA TFIIB (MIM 189963) TFIID (MIM 313650) TFIIE (MIM 189962) TFIIF (MIM 189968) TFIIG/TFIIJ and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520) respectively and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al. 1995 [PubMed 7724559]).[supplied by OMIM Mar 2008]

Gene Symbol:   GTF2A2
Gene Name:   general transcription factor IIA, 2, 12kDa
Aliases:   HsT18745, TF2A2, TFIIA
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000140307
Entrez:   2958
UniGene:   Hs.512934
Chromosome Mapping:   15q22.2

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