This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
Info: HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0026487; Intron-spanning
GDP-mannose 46-dehydratase (GMD; EC 184.108.40.206) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose the first step in the synthesis of GDP-fucose from GDP-mannose using NADP+ as a cofactor. The second and third steps of the pathway are catalyzed by a single enzyme GDP-keto-6-deoxymannose 35-epimerase 4-reductase designated FX in humans (MIM 137020).[supplied by OMIM Aug 2009]