PrimePCR™ ddPCR™ Copy Number Assay: VCP, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 60nt amplicon; intronic; HEX version of VCP (dHsaCP1000149)

 
Fluorophore:   HEX
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2507210
Chromosome Location:   hg199:35073021-35073143question
Amplicon Length:   60
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion 26S proteasome function and assembly of peroxisomes. This protein as a structural protein is associated with clathrin and heat-shock protein Hsc70 to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis including homotypic membrane fusion spindle pole body function and ubiquitin-dependent protein degradation. [provided by RefSeq Jul 2008]

Gene Symbol:   VCP
Gene Name:   valosin containing protein
Aliases:   IBMPFD, MGC131997, MGC148092, MGC8560, TERA, p97
RefSeq:   NC_000009.11 NG_007312.1 NG_007887.1 NT_008413.18
Ensembl:   ENSG00000165280
Entrez:   7415
UniGene:   Hs.529782
Chromosome Mapping:   9p13.3

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to TERT reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to TERT reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

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