This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
Info: HEX; 60nt amplicon; intronic; HEX version of VCP (dHsaCP1000149)
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion 26S proteasome function and assembly of peroxisomes. This protein as a structural protein is associated with clathrin and heat-shock protein Hsc70 to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis including homotypic membrane fusion spindle pole body function and ubiquitin-dependent protein degradation. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.
ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to TERT reference assay (HEX, Channel 2).