PrimePCR™ ddPCR™ Copy Number Assay: TOP2A, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 68nt amplicon; crosses exon-intron junction; HEX version of TOP2A (dHsaCP1000459)

 
Fluorophore:   HEX
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2507199
Chromosome Location:   hg1917:38574148-38574270question
Amplicon Length:   68
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

This gene encodes a DNA topoisomerase an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation chromatid separation and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form alpha is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq Jul 2010]

Gene Symbol:   TOP2A
Gene Name:   topoisomerase (DNA) II alpha 170kDa
Aliases:   TOP2, TP2A
RefSeq:   NC_000017.10 NG_027678.1 NT_010783.15
Ensembl:   ENSG00000131747
Entrez:   7153
UniGene:   Hs.156346
Chromosome Mapping:   17q21-q22

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis a single sample, using the EIF2C1 and TERT reference assays. Technical replicates are shown.

Copy Number Text

Copy Number
Copy number analysis a single sample, using the EIF2C1 and TERT reference assays. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to TERT reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to TERT reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

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