PrimePCR™ ddPCR™ Copy Number Assay: RUNX1, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 77nt amplicon; exonic; HEX version of RUNX1 (dHsaCP1000308)

 
Fluorophore:   HEX
List Price:    RMB0.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2507167
Chromosome Location:   hg1921:36259343-36259465question
Amplicon Length:   77
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RUNX1
Gene Name:   runt-related transcription factor 1
Aliases:   AML1, AML1-EVI-1, AMLCR1, CBFA2, EVI-1, PEBP2aB
RefSeq:   NC_000021.8 NT_011512.11 NG_011402.1
Ensembl:   ENSG00000159216
Entrez:   861
UniGene:   Hs.149261
Chromosome Mapping:   21q22.3

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

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