PrimePCR™ ddPCR™ Copy Number Assay: PTP4A3, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 67nt amplicon; exonic; HEX version of PTP4A3 (dHsaCP1000167)

 
Fluorophore:   HEX
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2507156
Chromosome Location:   hg198:142432326-142432448question
Amplicon Length:   67
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

The protein encoded by this gene belongs to a small class of prenylated protein tyrosine phosphatases (PTPs). PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. This class of PTPs contain a PTP domain and a characteristic C-terminal prenylation motif. Studies of this class of PTPs in mice demonstrated that they were prenylated proteins in vivo which suggested their association with cell plasma membrane. Overexpression of this gene in mammalian cells was reported to inhibit angiotensin-II induced cell calcium mobilization and promote cell growth. Two alternatively spliced variants exist. [provided by RefSeq Jul 2008]

Gene Symbol:   PTP4A3
Gene Name:   protein tyrosine phosphatase type IVA, member 3
Aliases:   PRL-3, PRL-R, PRL3
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000184489
Entrez:   11156
UniGene:   Hs.731499
Chromosome Mapping:   8q24.3

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to TERT reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to TERT reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

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