PrimePCR™ ddPCR™ Copy Number Assay: AKT2, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 70nt amplicon; crosses exon-intron junction; HEX version of AKT2 (dHsaCP1000418)

 
Fluorophore:   HEX
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506961
Chromosome Location:   hg1919:40762730-40762852question
Amplicon Length:   70
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq Jul 2008]

Gene Symbol:   AKT2
Gene Name:   v-akt murine thymoma viral oncogene homolog 2
Aliases:   PKBB, PKBBETA, PRKBB, RAC-BETA
RefSeq:   NC_000019.9 NG_012038.1 NT_011109.16
Ensembl:   ENSG00000105221
Entrez:   208
UniGene:   Hs.631535
Chromosome Mapping:   19q13.1-q13.2

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

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